Fig. 2
Ectopic expression of JAK2-V617F increases the kinetic sensitivity of PLCγ-1 (Tyr783) phosphorylation and of IP3R (Ser1756) phosphorylation upon EPO stimulation. (A) Western blot analysis of target proteins after 25 min of EPO (5 IU/ml) stimulation, following 16 h with or without EPO starvation. Numbers represent values normalized to the respective total protein. (B) Western blot analysis of targeted proteins at various times points after EPO (5 IU/ml) stimulation in 32D-JAK2-WT/EpoR and 32D-JAK2-V617F/EpoR cells. Bar diagrams, represent densitometric analysis of western blots, normalized to GAPDH and expressed as a ratio of phospho and total protein