Fig. 6

Administration of NRG1 down-regulates seizure susceptibility and rescues cognitive function of APP/PS1 mice. A Schematic diagram of intracerebroventricular injection. B-D The latency of seizure phenotypes (B), time to reach Racine V (C) and required dose of PTZ (D) in APP + PBS and APP + NRG1 acute kindling models. N = 3, 4. E Maximal Racine score reached every 5 min within 75 min. N = 3, 4. F Maximal Racine score reached per day. N = 13, 10. G Survival curve for PTZ chronic kindling model. N = 13, 10. H-J The mean speed (H), time in the corner zone (I) and time in the center zone (J) were recorded in the Open Field tests. N = 6, 7. K The percentage of time to explore new objects was recorded in the NOR tests. N = 6, 7. L The percentage of spontaneous alternation was recorded in the Y maze tests. N = 6, 7. M-Q In the MWM tests, the number of platform crosses (M), latency to platform (N), the mean swimming speed (O), time in the target quadrant (P) and latency to target quadrant (Q) were recorded during the probe test. R In the MWM tests, the escape latency was recorded in the acquisition trial. S Representative track images of mice in the probe test. N = 6, 7. T-V Western blot and quantitative analysis for synapse-associated proteins in the hippocampus, including PSD-95 (U) and Syn-1 (V). N = 5. Data were shown as means ± SEM. Two-way ANOVA for (E, F, R). Mann Whitney test for (D, P). Unpaired t test for (B, C, H-O, Q, U, V). Log-rank test for (G). *p < 0.05, ** p < 0.01, ***p < 0.001