Fig. 5

Impaired GABAergic inputs in the hippocampus of APP/PS1 mice. A Representative AP traces of evoked spikes. B Average AP firing frequency of CA1 pyramidal neurons in response to 0- to 200-pA depolarizing current steps. N = 3 mice per group, n = 6–7 neurons per mouse. C Evoked threshold of CA1 pyramidal neurons of APP and WT mice. N = 3 mice per group, n = 6–7 neurons per mouse. D Rest membrane potential of CA1 pyramidal neurons of APP and WT mice. N = 3 mice per group, n = 6–7 neurons per mouse. E Representative traces of mIPSC recordings in hippocampal CA1 region. F, G Mean mIPSC amplitude (F) and frequency (G) in CA1 pyramidal neurons. N = 3 mice per group, n = 3–5 neurons per mouse. H Representative fluorescence images showing the PV⁺ Ins (green) and SOM⁺ Ins (red) in the hippocampal CA1 region of APP and WT mice. Scale bar, 50 μm. I Comparable number of PV⁺ Ins in the hippocampal CA1 region of APP and WT mice. N = 3 mice per group, n = 6 (average of 12 slices) per mouse. J Comparable number of SOM⁺ Ins in the hippocampal CA1 region of APP and WT mice. N = 3 mice per group, n = 6 (average of 12 slices) per mouse. K-P Western blot and quantitative analysis for vGAT (L), GAD65/67 (M), NRG1 (O) and ErbB4 (P). N = 3. Q, R Quantitative analysis for number of ErbB4⁺ cells per unit area (Q), and mean fluorescent intensity of ErbB4 particularly in PV⁺ Ins (R). N = 3 mice per group, n = 3 (average of 9 slices, 72–81 cells) per mouse. S Representative fluorescence images showing the PV⁺ Ins (green) and ErbB4 (red) in the hippocampal CA1 region of APP and WT mice. Scale bar, 50 μm. Data were shown as means ± SEM. Two-way ANOVA for (B). Mann Whitney test for (C). Unpaired t test for (D, F, G, I, J, L, M, O-R). *p < 0.05, ** p < 0.01, ***p < 0.001