Fig. 4

Effect of acidic media on differentiation markers in HK-2 and CCD-1092Sk cells in mono-and coculture. Protein expression changes of ß-catenin, N-cadherin, vinculin and vimentin in HK-2 in mono (a-b, m–o) coculture (d-f, p-r) and CCD-1092Sk in mono (g-i, s-u) and coculture (j-l, v-x). Representative western blots of proteins isolated from cells exposed to acidosis (y–z). n = 8—18. Exposure time = 48 h