Fig. 10
Effect of acidic media on the expression of MKK3 and DUSP10 in HK-2 and CCD-1092Sk cells in coculture. Impact of acidic media on the expression of MKK3 in HK-2 cells after 3 h (a-c) and after 48 h (d-f). Impact of acidic media on the expression of MKK3 in CCD-1092Sk cells after 3 h (g-i) and after 48 h (j-l). Effect of acidic media on DUSP10 expression HK-2 and CCD-1092Sk cells after 3 h (o, q) and after 48 h (p, r). Comparison of the acidosis-effect in HK-2 and CCD-1092Sk cells on MKK3 (8s) and DUSP10 (8t) Representative western blots of proteins isolated from cells exposed to acidosis (m, n) n = 6–9. exposure time = 3h or 48 h