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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Pan-histone deacetylase inhibitor vorinostat suppresses osteoclastic bone resorption through modulation of RANKL-evoked signaling and ameliorates ovariectomy-induced bone loss

Fig. 6

Molecular docking results of the SAHA–RANKL interaction. A Structural 3D image of RANK/RANKL complex. B Diagram of the binding capacity of molecular SAHA to the cavity on the RANKL protein surface. C A 2D interaction diagram of compound SAHA with RANKL. Molecular SAHA is surrounded by amino acids. The red arcs represent the sites where the protein amino acids form hydrophobic interactions with SAHA, while the sites forming hydrogen bonds exhibit complete amino acid structures. D Image in secondary structure indicating hydrogen bond interactions between SAHA and amino acid residues of RANKL. E RANKL concentration in cell culture supernatants after incubation with different doses of SAHA (0, 0.5, 1 μM) at the initial RANKL concentration of 50 ng/ml. F Representative images of western blotting for TRAF6 expression. G Quantitative analysis of TRAF6 normalized to β-actin. All data are shown as mean ± SD, n = 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X