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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Pan-histone deacetylase inhibitor vorinostat suppresses osteoclastic bone resorption through modulation of RANKL-evoked signaling and ameliorates ovariectomy-induced bone loss

Fig. 4

SAHA inhibits NFATc1 signaling. A The expression of NFATc1, MMP9 and Cathepsin K proteins. B-D Quantitative statistics of NFATc1, MMP9 and Cathepsin K expression normalized to β-actin expression. E Representative images of immunofluorescence staining indicating the effect of SAHA on RANKL-induced activity of NFATc1 in RAW 264.7 cells (Scale bar = 100 μm). NFATc1 (red color), F-actin (green color) and nuclei (blue color). F-G Quantification of the mean fluorescence intensity of NFATc1 and the nuclei number per osteoclast. H Representative images of immunofluorescence staining indicating the effect of SAHA on RANKL-induced activity of NFATc1 in BMDM (Scale bar = 100 μm). NFATc1 (red color), F-actin (green color) and nuclei (blue color). I-J Quantification of the mean fluorescence intensity of NFATc1 and the nuclei number per osteoclast. All data are shown as mean ± SD, n = 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001, relative to the positive control group (treated with RANKL but without SAHA)

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Cell Communication and Signaling

ISSN: 1478-811X