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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Pan-histone deacetylase inhibitor vorinostat suppresses osteoclastic bone resorption through modulation of RANKL-evoked signaling and ameliorates ovariectomy-induced bone loss

Fig. 2

SAHA impedes F-actin belt formation and osteoclastic bone resorption. A Representative fluorescence micrographs for F-actin belt formation after SAHA addition in RAW 264.7 cells (Scale bar = 100 μm). F-actin (green color) and nuclei (blue color). B-C Quantitative statistics of the nuclei number per osteoclast and mean area of osteoclasts. D Representative fluorescence micrographs for F-actin belt formation after SAHA addition in BMDM (Scale bar = 100 μm). F-actin (green color) and nuclei (blue color). E-F Quantitative statistics of the nuclei number per osteoclast and mean area of osteoclasts. G Representative images of TRAcP staining for osteoclast maturation after treating SAHA in RAW 264.7 cells (Scale bar = 100 μm) and electron microscope scanning of bovine bone slices resorption pits (Scale bar = 100 μm). H-I Quantification of the average area of bone slice resorption and the area of resorption per osteoclast in each group. J Representative images of TRAcP staining for osteoclast maturation after treating SAHA in BMDM (Scale bar = 100 μm) and electron microscope scanning of bovine bone slices resorption pits (Scale bar = 100 μm). K-L Quantification of the average area of bone slice resorption and the area of resorption per osteoclast in each group. Bar graphs are indicated by mean ± SD, n = 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001, in comparison to the positive control group (treated with RANKL but without SAHA)

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Cell Communication and Signaling

ISSN: 1478-811X