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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Pan-histone deacetylase inhibitor vorinostat suppresses osteoclastic bone resorption through modulation of RANKL-evoked signaling and ameliorates ovariectomy-induced bone loss

Fig. 1

SAHA restrains RANKL-induced osteoclast differentiation. A The chemical structure and molecular formula of SAHA. B-C The effects of distinct SAHA doses on RAW 264.7 cell viability for 72 h as evaluated by CCK-8 assay. D Representative images of TRAcP staining visualizing the formed osteoclasts treated with SAHA at indicated doses (Scale bar = 100 μm). E Representative TRAcP staining images displaying osteoclasts treated with 1 μM SAHA at different stages (Scale bar = 100 μm). F-G Quantification of TRAcP+ multinucleate cells (nuclei > 3) per field and the average area of osteoclasts per group in Fig. 1D. H-I Quantitative analysis of TRAcP+ multinucleated cells with nuclei count greater than 3 and area of osteoclasts in Fig. 1D. Bar graphs are shown as mean ± SD, n = 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001 and ns: no significance, as compared to the positive control group (treated with RANKL but without SAHA)

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Cell Communication and Signaling

ISSN: 1478-811X