Fig. 5
From: Hypoxic glioblastoma-cell-derived extracellular vesicles impair cGAS-STING activity in macrophages
Hypoxic GBM cells deliver miR-25/93 to macrophages via hypoxic GBM-derived EVs. A., B. BMDMs were incubated for 24 h under hypoxia (1% O2) or normoxia (21% O2) with addition of EVs derived from hypoxic (1% EVs) or normoxic (21% EVs) SB28 cells. Total miRNA was extracted from the BMDMs and the levels of miR-25 (A) and miR-93 (B) were measured by RT-qPCR. C., D. BMDMs were incubated for 24 h under hypoxia (1% O2) or normoxia (21% O2) with addition of EVs derived from hypoxic (1% EVs) or normoxic (21% EVs) miR25/93 KO SB28 cells. Total miRNA was extracted from the BMDMs and the levels of miR-25 (C) and miR-93 (D) were measured by RT-qPCR. Values are expressed as mean ± SD of three biologic replicates, and comparisons were made using an unpaired t test. **p < 0.005, ***P < 0.001