Fig. 4
From: Hypoxic glioblastoma-cell-derived extracellular vesicles impair cGAS-STING activity in macrophages
Hypoxia upregulates mir25/93 expression in human and mouse GBM cells and derived EVs. A Cellular expression levels of miR-25 (left) and miR-93 (right) in human (Ge904) and mouse (SB28) GBM cells measured by RT-qPCR. miR-191 was used as stably expressed housekeeping miRNA normalization control. B EVs secreted from human (Ge904) and mouse (SB28) GBM cells cultured for 24 h in hypoxic (1% O2) or normoxic (21% O2) conditions were analyzed for miR-25 (left) and miR-93(right) levels by RT-qPCR. cel-miR-39 spike-in control was added as a normalization control. Data is presented as the mean ± SD of three independent experiments and comparisons were made using an unpaired t test. *p < 0.05, **p < 0.005, ***P < 0.001