Fig. 3
From: Hypoxic glioblastoma-cell-derived extracellular vesicles impair cGAS-STING activity in macrophages
GBM-derived EVs are internalized by macrophages. A Kinetics of macrophage uptake of BODIPY membrane-labelled (red) EVs derived from hypoxic SB28 cells at the indicated times. EPMs were stained for F4/80 (green) and DAPI (blue). Negative control: macrophages incubated with addition of non-labelled EVs. For passive membrane uptake control, macrophages were incubated at 4 °C for 6 h with BODIPY membrane-labelled EVs. B Uptake of hypoxic SB28-derived EVs labeled with BODIPY (red) by BMDMs, M0, or after polarization to M1 and M2. BMDMs were incubated with labeled EVs for 24 h at 37 °C and stained for CD11b (green) and DAPI (blue). C Uptake of BODIPY-labeled (red) EVs from hypoxic Ge835 or Ge904 cells by human monocyte-derived macrophages (MDMs) stained for CD68 (green) and DAPI (blue). Control: MDMs without EVs. MDMs were incubated with the corresponding labeled EVs for 24 h at 37 °C before fixation and imaging. Pictures are representative of a minimum of 3 images per group