Fig. 2
From: Hypoxic glioblastoma-cell-derived extracellular vesicles impair cGAS-STING activity in macrophages
NTA characterization of GBM-derived EVs from normoxic or hypoxic culture. A NTA profiles of EVs isolated from murine SB28 and GL261 cell lines (left) and from human Ge904, Ge835 and LN18 GBM cell lines (right) cultured in hypoxic (1% O2) or normoxic (21% O2) conditions. (upper panels). Total number of EVs measured by NTA from murine (SB28 and GL261) (left) and from human (Ge904, Ge835 and LN18) GBM cell lines (right) cultured in hypoxic (1% O2) or normoxic (21% O2) conditions (lower panel). EV depleted culture media was used as control. The calculated size distribution in the upper panels is depicted as a mean from three experiments and three measurements. In the lower panels, data is presented as the mean ± SD of three independent experiments and comparisons were made using an unpaired t test. *p < 0.05, **p < 0.005, ***P < 0.001. B fNTA profiles of EVs isolated from murine SB28 and GL261 cell lines (left) and from human Ge904, Ge835 and GBM cell lines (right) cultured in hypoxic (1% O2) or normoxic (21% O2) conditions. The EVs were stained with APC conjugated anti-CD9 antibody (anti-mouse or anti-human correspondingly). EV depleted culture media was used as control. The calculated size distribution is depicted as a mean from three experiments and three measurements. C Western blot analysis of cells and EVs from murine (SB28) and human (Ge904 and Ge835) cell lines