Fig. 2

ZEB1 reporter expression and Anti-ZEB1 immunocytochemistry. A Fluorescence representation of mean ZEB1 reporter intensity and mean ZEB1 ICC in BCPAP WT and BCPAP CD73- cells. B Violin plots illustrating cell distribution based on mean ZEB1 reporter intensity and mean ZEB1 ICC. C Bar plots of ZEB1 reporter expression from flow cytometry. Expression of ZEB1’s non-coding RNA regulation reporter (GFP/FITC) is presented based on mean values of the triplicate. *** = p ≤ 0.001; **** = p ≤ 0.0001; ns = not significant. D Representative Western blot showing the expression levels of ZEB1 protein in lysates of cell lines derived from PTC. E Densitometric analysis of the Western blot. Band intensities were quantified using ImageJ software. The expression levels of ZEB1 protein were normalized to the total protein stain from respective lanes