Fig. 6

The activation of NCOA4-FTH ferritinophagy axis in paraquat-treated cells. A, B Lung epithelial cells were subjected to PQ treatment with concentration gradient or time gradient, and followed by Western blot. C The representative images of RPA staining in lung epithelial cells treated with PQ in the presence or absence of CQ (25 μM); D Western blot analysis of the indicated protein levels in lung epithelial cells treated with designed treatment; β-actin was used as loading control; E Flow cytometric analysis of cellular ROS through the staining of DCF-DA. F FTH enforced expression cells were transfected with LC3-GFP, then the colocalization of FTH and autophagosome was analyzed by confocal microscope. Scale bar: 20 μm. ^★★P < 0.01 between indicated groups