Fig. 4

Paraquat enhances the activity of lysosomes in human respiratory epithelial cells. A Gene set enrichment analysis (GSEA) indicating the hallmarks of lysosome were enriched in the PQ-treated group; B The representative images of Lyso-Tracker staining in cells transfected with LC3-GFP after the treatment of vehicle or 500 μM PQ for 4 h; C The representative images of Magic Red™ cathepsin B staining in cells transfected with LC3-GFP after the treatment of vehicle or 500 μM PQ for 4 h. DAPI was used for nuclear staining; Scale bar: 20 μm. Fluorescence analysis of the lysosomal activity through the staining of Lyso-Tracker (D, E) and Magic Red™ cathepsin B (F, G) with designed treatment for 4 h. ^★★P < 0.01 between indicated groups