Fig. 2

Paraquat induces ferroptotic cell death of human respiratory epithelial cells. A KEGG analysis of the differentially expressed genes between the BEAS-2B cells with or without PQ treatment; B The heatmap of ferroptosis related gene expression. C Enrichment plots of ferroptosis from GSEA. The statistical results of cell survival assay (D) and LDH release levels (E) of BEAS-2B cells treated with or without the indicated chemicals (0.5 mM paraquat, 1 μM ferrostatin-1, 100 μM DFO, 0.5 mM GSH, 0.5 mM NAC, 5 μM Z-VAD-FMK, 0.5 μM Necrosulfonamide) for 12 h; F The relative RPA fluorescence in the indicated treatment groups (Paraquat: 0/125/250/500 μM; DFO: 100 μM); G The relative GSH levels in the indicated treatment groups ((Paraquat: 0/125/250/500 μM; BSO: 100 μM); All histograms were represented as mean ± SD. ^★P < 0.05, ^★★P < 0.01 between indicated groups