Fig. 5

Rolapitant facilitated the activation of DR5 signaling pathway and apoptosis of lung cancer cells by up regulating CHOP. A, B DR5 and CHOP expression was confirmed in A549, H1299 and H460 cells treated with DMSO or indicated concentrations (10, 15, 20, or 25 μM) of Rolapitant for 48 h by qRT-PCR. C Western blot analysis of CHOP, DR5, DR4, Caspase-8, Cleaved-Caspase-8, Caspase-3, Cleaved-Caspase-3 and FADD levels in A549 and H1299 cells treated with DMSO or indicated concentrations (10, 15, 20, or 25 μM) of Rolapitant for 48 h. GAPDH was used as a loading control. D Western blot analysis of the three independent CHOP siRNA knockdown effects. E, F CHOP siRNA knocking down and control A549 and H1299 cells were treated with DMSO or Rolapitant (10 μM) for 48 h. The expression of Caspase-8, Cleaved-Caspase-8, Caspase-3, Cleaved-Caspase-3, PARP and Cleaved-PARP were determined by western blot