Fig. 7

STAT3 inhibitor attenuates cell proliferation and migration induced by ERp57. A STAT inhibitor abrogated cell proliferation induced by ERp57. Cell growth of HT1080 cells was measured by CCK-8 assay on day 0, 1, and 2 after incubation with 30 nM EGF, 50 nM ERp57 or ERp57ΔQEDL, in the absence or presence of 4 μM cryptotanshinone, in MEM supplemented with 10% FBS. OD450 measurements of cells at indicated time points were represented by histogram. Statistical analysis was performed using two-way ANOVA with a Dunnett’s post-hoc test for multiple comparisons. n = 3 independent experiments. **: P < 0.01.****: P < 0.0001. B, C STAT3 inhibitor suppressed cell migration in wound healing assay. Phase contrast images of HT1080 cells incubated with indicated reagents at different time points after wounding (B). The migration distance in wound healing was statistically analyzed using two-way ANOVA with a Dunnett’s post-hoc test for multiple comparisons (C). n = 4 independent experiments. **: P < 0.01. ***: P < 0.001.****: P < 0.0001. ns: not significant. Scale bar = 300 μm