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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: High estrogen during ovarian stimulation induced loss of maternal imprinted methylation that is essential for placental development via overexpression of TET2 in mouse oocytes

Fig. 4

Effect of β- estradiol (E2) on oocytes DNA methylation and associated proteins. A, B Representative immunofluorescence images of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in germinal vesicle (GV) and metaphase II (MII) oocytes, showing red fluorescence with Propidium Iodide (PI) staining and green fluorescence with Dylight 488 staining, white circle indicates the first polar body. C, D Quantification of average fluorescent intensities with 5mC and 5hmC staining in each group (n = 30). E, F Analysis of RNA levels for ten-eleven translocation 2 (Tet2, n = 8), estrogen receptor alpha (ERα, n = 8) and mesoderm-specific transcript homologue (Mest, n = 8) in each group. Data are expressed as the means ± standard deviation (SD), *P < 0.05, **P < 0.01, ***P < 0.001 (One-way ANOVA multiple comparisons test). Scale bar = 30 μm

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Cell Communication and Signaling

ISSN: 1478-811X