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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: High estrogen during ovarian stimulation induced loss of maternal imprinted methylation that is essential for placental development via overexpression of TET2 in mouse oocytes

Fig. 1

Effect of ovarian stimulation (OS) on relative intensity of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) fluorescence of oocytes and pronuclear-stage embryos. A, C Representative immunofluorescence images of 5mC and 5hmC in germinal vesicle (GV) and metaphase II (MII) oocytes, red fluorescence with Propidium Iodide (PI) staining and green fluorescence with Dylight 488 staining, white circle indicates the first polar body. B, D Quantification of average fluorescent intensities with 5mC and 5hmC staining within each group (n = 30). E Representative immunofluorescence images of 5mC and 5hmC in pronuclear-stage embryos, white circle indicates the second polar body. F, G Quantification of average fluorescent intensities with 5mC and 5hmC staining of female and male pronucleus (n = 20). H Relative mRNA level of Analysis of RNA levels for DNA methyltransferase 1, 3a and 3b (Dnmt1, Dnmt3a, Dnmt3b, n = 8). I-J Expression level of ten-eleven translocation 1,2 and 3 (Tet1, Tet2, Tet3) and estrogen receptor alpha (ERα) within each group (n = 8). Data are expressed as the means ± standard deviation (SD), ns: Not Statistically Significant, *P < 0.05, **P < 0.01, ***P < 0.001(unpaired two-tailed t test). Scale bar = 30 μm

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Cell Communication and Signaling

ISSN: 1478-811X