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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Dopamine enhances recovery after traumatic brain injury through ubiquitylation and autophagic degradation of RIPK1

Fig. 3

DRD1 signaling promotes RIPK1 degradation via autophagy. A-C Samples from patients with severe TBI were analyzed. The level RIPK1 was determined by immunoblotting (A and B) and immunohistochemistry (C). D and E Immunoblot analysis of RIPK1 and b-actin in cell lysates from primary neurons treated with MG132 (20μM) for 30 min and then stimulated with A-68930 (20μM) for 3 h (n=5/group). F and G Immunoblot analysis of RIPK1 and b-actin in cell lysates from primary neurons treated with 3-MA (2mM) for 30 min and then stimulated with A-68930 (20μM) for 3 h (n=5/group). H and I Immunoblot analysis of LC3, RIPK1, and b-actin in primary neurons from beclin1-/+ mice treated with A-86930 (20μM) for 3 h (n=5/group). J and K Immunofluorescence of LC3 (green) and RIPK1 (red) in brain tissue from mice, which were pre-treated by BAF (n=5/group). L and M Immunofluorescence of LAMP1 (green) and RIPK1 (red) in brain tissue from mice (n=5/group)

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Cell Communication and Signaling

ISSN: 1478-811X