Fig. 2

DA inhibits neural death via DRD1 in the model of stretch injury. A Representative photomicrographs of NeuN (red) and Map2 (green) expressing primary cortical neurons utilized in this study. B and C Primary neurons were subjected to stretch injury and then treated with DA (0.2mM) for 3 h. Representative photomicrographs of NeuN (red) and cleaved-caspase-3 (green) (n  = 3/group). D and E Primary neurons were subjected to stretch injury and then treated with DA (0.2mM) for 3 h. LDH release (D) and cell death (E) were measured by LDH assay and SytoxGreen positivity, respectively (n = 5/group). F Quantification of cell death from primary neurons which were transfected with control siRNA with a scrambled sequence or Drd1-Drd5 -specific siRNA as indicated, subjected to stretch injury and then treated for 3 h with DA (0.2mM). Cell death was measured by SytoxGreen positivity (n = 5/group). G and H Primary neurons from Drd1 +/+ and D rd1 −/− mice were subjected to stretch injury and then treated for 3 h with DA (0.2mM). LDH release (G) and quantification of cell death (H) were measured by LDH assay and SytoxGreen positivity, respectively (n = 5/group). Data were represented as mean ± SD. NS, no significance