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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: Identification of myoferlin as a mitochondria-associated membranes component required for calcium signaling in PDAC cell lines

Fig. 8

Myoferlin interacts with IP3R3. A IP3R3 was immunoprecipitated from Panc-1 and MiaPaCa-2 cell lines. Myoferlin co-immunoprecipitation was assessed by western blot. The IP3R3-myoferlin co-immunoprecipitation is representative of three independent experiments. B Myoferlin and IP3R3 immunofluorescence in Panc-1 and MiaPaCa-2 cell lines. Scale bars = 20 μm or 5 μm in high magnification. Confocal pictures were acquired with a high resolution LSM 880 microscope. C Colocalization analyses using Manders’ method on both Panc-1 and MiaPaCa-2 cell lines. “IP3R3 ϵ Myoferlin” represents the proportion of above-threshold pixels in IP3R3 channel colocalizing with above-threshold pixels in myoferlin channel and vice versa for “Myoferlin ϵ IP3R3”. Results are presented as mean ± SD. The number of analyzed cells was 27 for Panc-1 cells, and 20 for MiaPaCa-2. The pictures are representative of at least two independent experiments

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Cell Communication and Signaling

ISSN: 1478-811X