Fig. 6

PLA between VDAC1 and IP3R3. A Representative pictures for PLA between VDAC1 and IP3R3 in PDAC cell lines silenced for myoferlin. The pictures were acquired with a confocal Nikon A1R microscope. B Quantification of PLA dots per cells in Panc-1 and MiaPaCa-2 cell lines. The number of pictures, from three independent experiments, analyzed for the PLA in the Panc-1 cell line was n = 33 (irrelevant), n = 19 (Myof#1 siRNA), n = 30 (Myof#2 siRNA), n = 27 (no siRNA). The number of pictures, from three independent experiments, for the PLA in the MiaPaCa-2 cell line was n = 20 (irrelevant), n = 20 (Myof#1 siRNA), n = 20 (Myof#2 siRNA) and n = 19 (no siRNA). Results were presented as mean ± SD. The non-parametric Kruskal-Wallis test was used for statistical analysis. **** p-value < 0.0001. ns: non-significant. Western blot inserts validate myoferlin silencing. HSC70 was used as a loading control. The same batch of transfected Panc-1 cells was used in Fig. 5C