Fig. 5

Myoferlin silencing does not impact abundance of MAMs-related proteins upon myoferlin silencing and the contacts between ER and mitochondria. A TEM pictures representing MAMs in Panc-1 and MiaPaCa-2 cell lines. MAMs, highlighted by black arrows on the upper panels, are shown at high magnification on the lower panel. Scale bars = 5 μm, except for high magnification, where scale bars = 0.2 μm. B Graphs showing the ERMICC values from controls and myoferlin-silenced cells in Panc-1 and MiaPaCa-2 cell lines. The non-parametric Kruskal-Wallis test was used for statistical analysis. For the Panc-1 cell line, the number of mitochondria in each condition was n = 298 (16 pictures, irrelevant), n = 138 (7 pictures, Myof#1 siRNA), n = 122 (5 pictures, Myof#5 siRNA) and n = 93 (6 pictures, no siRNA). Regarding the MiaPaCa-2 cell line, the number of mitochondria was n = 210 (10 pictures, irrelevant), n = 224 (9 pictures, Myof#1 siRNA), n = 174 (11 pictures, Myof#5 siRNA) and n = 189 (9 pictures, no siRNA). ns = non-significant; *: p-value < 0.05; **: p-value < 0.01. Results were presented as mean ± SEM. C Myoferlin, MFNs, S1R, IP3R3, VDAC1, GRP75 and MCU from whole cell lysates were assessed by western blot. The quantifications were performed with ImageJ software [24]. The irrelevant siRNA condition was used as reference for the quantifications. HSC70 was used as a loading control. The same batch of transfected Panc-1 cells was used in Fig. 6. Western blots are representative of three biological replicates