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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: The dynamic role of nucleoprotein SHCBP1 in the cancer cell cycle and its potential as a synergistic target for DNA-damaging agents in cancer therapy

Fig. 3

SHCBP1 dynamically localizes to centrosomes and different spindle sites during cell mitosis. A Immunofluorescence analysis of SHCBP1 localization (red) throughout the cell cycle (interphase; early and late prophase; prometaphase; metaphase; early, middle, and late anaphase; and telophase) as assessed by co-staining with γ-tubulin (green) and Hoechst (blue). White boxes mark the centrosome in the interphase or after cytokinesis; white-arrowheads indicate the spindle poles during the mitosis; yellow-arrowheads point to the central spindle; white arrows: midbody. Scale bars, 20 μm. B Immunofluorescence co-staining of SHCBP1 (green) and α-tubulin (red) in NSCLC cells. White boxes: centrosomes; white arrows: midbody. Scale bars, 20 μm. C Immunofluorescence co-staining of SHCBP1 (green) and α-tubulin (red) at the midbody. Scale bars, 8 μm. D Immunofluorescence co-staining of SHCBP1 (green) and PLK1 (red) or RACGAP1 (red) in NSCLC cells. White arrows indicate midbody. Scale bars, 10 μm

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Cell Communication and Signaling

ISSN: 1478-811X