Fig. 1

High expression of SHCBP1 in LUAD tissues was associated with poor prognosis. A SHCBP1 expression in paired tumour and normal tissues in pan-cancer data of TCGA. The Oncomine dataset (left panel) represents the expression of SHCBP1 in the existing pan-cancer database. The selected criteria were fold change > 2 and p-value < 0.0001. The SHCBP1expression in 37 kinds of tumour types with or without adjacent normal tissues determined by TIMER from TCGA (right panel). Statistical significance is represented as *, P < 0.05; **, P < 0.01; ***, P < 0.001. Boxplots indicate median, lower and upper quartile. B SHCBP1 mRNA expression in paired LUAD and normal tissues (n = 57) from the TCGA database. Paired t-test was used for the analysis; ****, P < 0.0001. C Correlation analysis of mRNA expression level between SHCBP1 and MKI67 or NKX2-1 in LUAD tissues from TCGA database (n = 504). Pearson correlation analysis provides correlation coefficient (r) and P -value. D, E GO (D) and KEGG (E) enrichment analysis of 346 genes significantly correlated with SHCBP1 expression (p < 0.0001, r ≥ 0.5) in LUAD tissues (data downloaded from online database, http://www.cbioportal.org/). The enrichment analysis was performed by using the R package cluster Profiler. Only the top 10 scoring pathways are presented. F, G Kaplan–Meier overall survival curves of LUAD patients with high or low SHCBP1expression (stratified by median value) (F) and LUAD patients receiving radiotherapy with high or low SHCBP1expression (stratified by median value) (G). Data was downloaded from http://www.oncolnc.org/. p values were determined by log-rank test. HR, hazard ratio. H Western blot analysis of SHCBP1 expression in tumour tissues and paired normal tissues taken from 81 patients with surgically resected LUAD. T, tumour tissue; ANT, adjacent normal tissue. I SHCBP1 mRNA level in tumour tissues and paired normal tissues taken from 77 patients with surgically resected LUAD detected by Real-time quantitative PCR (qRT-PCR). Line graph analyzed by paired t-test, each data point represents the mean value of three technical replicates of one sample. T, tumour tissue; ANT, adjacent normal tissue. J Representative immunohistochemistry images of SHCBP1 and Ki67 staining in different clinical stages of patients with LUAD. Scale bars, 100um. The area surrounded by a box in stage III was magnified, which highlights the SHCBP1 staining in tumour cells. K Representative immunofluorescence images of SHCBP1 staining in two patients with LUAD. Scale bars, 50um. The area surrounded by a box was magnified. L, M Kaplan–Meier overall survival and progression-free survival curves of patients with surgically resected NSCLC (L) or LUAD (M) with high or low SHCBP1 expression. SHCBP1 expression was stratified by the immunohistochemistry staining score (high, score = 12; low, score < 12). N, O Immunohistochemical staining (N) and Kaplan–Meier survival curves (O) of SHCBP1 expression in tissue microarrays from 93 patients with LUAD from another hospital. #, refers to the last 6 patients without ANT. T, tumour tissue; ANT, adjacent normal tissue