Fig. 3

Decreased CSCs in KMT2B and KMT2D KD reduced CSCs but increased BC late-progenitor. A MDA-MB-231 BCCs with the Oct4a-dsRED reporter vector and transduced with the KMT2D-GFP shRNA were subjected to flow cytometry to measure the relative dsRED intensity. B Percent distribution of BCC subsets after transduction with scramble-shRNA or KMT2D shRNA. C Total number of CSCs (Oct4a^high) in scramble BCCs and KMT2D knockdown BCCs. Each experiment was repeated thrice, where a *p < .05 was considered significant. D Reat time PCR for stem cell-associated genes in KMT2B and KMT2D KD BCCs. Control PCR used cDNA from BCCs with scramble shRNA. The value for scramble shRNA is assigned 1 and the experimental values are presented as the fold change. The results show three biological replicates, mean ± SD, *p < .05 vs. scramble shRNA