Fig. 8
Mitochondria-Derived Vesicles influence chemotherapy response. A A2780 cells were incubated with media or conditioned media (CM) as indicated (A2780 CIS CM, A2780 CIS EVs, A2780 CIS CM w/o EVs, A2780 CIS GW4869 CM, and A2780 EVs), and were treated with stepwise concentrations of CDDP. Then, cell viability was measured with the MTT assay. B EC50 was determined in A2780 cells treated as indicated after 72 h of incubation with CDDP using the MTT assay and calculated with GraphPad Prism. C Lysates from A2780 cells incubated with media or conditioned media (CM) as indicated (A2780 CIS CM, A2780 CIS EVs, A2780 CIS CM w/o EVs, and A2780 CIS GW4869 CM) were analyzed by immunoblotting using antibodies against RAB7 and HSP90, as the loading control. D Densitometric analysis was performed by Image Lab software (Bio-Rad). E A2780 cells were incubated with media or conditioned media (CM) as indicated (A2780 EVs and A2780 CCCP EVs CM) and were treated with stepwise concentrations of CDDP. Then cell viability was measured with the MTT assay. F EC50 was determined in A2780 cells treated as indicated after 72 h of incubation with CDDP using the MTT assay and calculated with GraphPad Prism. G Live imaging assay using LysoTracker DND-26 and MitoTracker Red CM-H2XRos on A2780 cells incubated with A2780 CIS CM and A2780 CIS EVs and on A2780 CIS cells used as control. H-I Number of lysosomes for cells and intensity of MitoTracker Red CM-H2XRos were determined by ImageJ software. Data represent the mean ± SEM of at least three independent experiments. * p < 0.05; ** p < 0.01; ***p < 0.001. Scale bar = 10 μm