Fig. 1

Impairment of the endocytic pathway in chemoresistant cells. A EC50 was determined in A2780 and in A2780 CIS cells after 72 h of treatment with CDDP using the MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenol tetrazolium bromide) assay. B, C Relative protein abundance of RAB4, RAB5A, RAB7, RAB9, RAB27, RILP, LAMP-1, and ATP6V1G1 was assessed by Western blot analysis and quantified by densitometry normalizing against α-Tubulin. D Late endocytic acid compartments were live stained with LysoTracker DND-99 dye and images were acquired with confocal microscopy. E, F The number and size of LysoTracker-positive organelles were determined by ImageJ software. G Quantification of Green DQBSA puncta per cell was determined by ImageJ software. Data represent the mean ± SEM of at least three independent experiments. * p < 0.05; ** p < 0.01; ***p < 0.001