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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: Characterization of two distinct immortalized endothelial cell lines, EA.hy926 and HMEC-1, for in vitro studies: exploring the impact of calcium electroporation, Ca2+ signaling and transcriptomic profiles

Fig. 8

Comparison of global transcriptomic profiles of EA.hy926 and HMEC-1 cells. A PCA plot showing clustering of EA.hy926 and HMEC-1 samples based on normalized gene expression levels. PC1 - principal component 1, PC2 - principal component 2. B Heatmap presenting unsupervised hierarchical clustering of all genes across EA.hy926 and HMEC-1 samples. Normalized expression levels were scaled to Z-scores for each gene. C Volcano plot showing DEGs in EA.hy926 samples compared to HMEC-1 samples. Genes with adjusted P values of < 0.05 and |log2FC| > 1 were considered to be statistically significant. A total of 16,283 genes were analyzed. NS - not significant, FC - fold change. D-F Dot plots presenting the top 10 significantly enriched GO processes based on hitsPerc (n (DEGs in category)/n (all genes in category) * 100). A P value < 0.05 was considered significant. BP - biological processes (D), CC - cellular components (E), MF - molecular functions (F). Five replicates per group in all analyses are presented

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Cell Communication and Signaling

ISSN: 1478-811X