Fig. 7

Fluorometric kinetic measurements of [Ca²⁺]_i in EA.hy926 and HMEC-1 cells. A) Time course of the changes in the F₃₄₀/F₃₈₀ ratio in EA.hy926 and HMEC-1 cells during the injection of ATP (100 μM) with or without preincubation with thapsigargin (TG) (1 μM). These changes were followed in the presence or absence of Ca²⁺ (no Ca²⁺). Data are presented as AM ± SEM (n ≥ 9). B) Summary bar graph showing the average maximum increase in the F₃₄₀/F₃₈₀ ratio in HMEC-1 and EA.hy926 cells treated as described in A). Bars display AM of maximum amplitude in F₃₄₀/F₃₈₀ ratio ± SE (n ≥ 9). Statistical significance was determined using a nonparametric t-test (Mann-Whitney). A P value of < 0.05 was considered to be statistically significant (*P < 0.05 vs Ca²⁺ ATP, **P < 0.05 between EA.hy926 and HMEC-1 cells). C) Time course of the changes in the F₃₄₀/F₃₈₀ ratio in EA.hy926 and HMEC-1 cells during the injection of bradykinin (10 μM) alone or bradykinin (10 μM) with preincubation with LaCl₃ (500 μM) or GdCl₃ (500 μM)