Fig. 5
Effects of increased intracellular Ca2+ concentration by CaEP on cell–cell junctions in EA.hy926 and HMEC-1 cells. A Representative confocal images show changes in the cell–cell adhesion protein VE-cadherin in EA.hy926 and HMEC-1 cells at 1, 4 and 24 h after EP alone or after CaEP in the presence of 0.5, 2 or 3 mM Ca2+. B The plots represent quantification of the changes in average VE-cadherin mean fluorescence intensity after CaEP at 1, 4 and 24 h time points. Data were normalized to the control and presented as AM ± SEM (n ≥ 3). Statistical significance was determined using a nonparametric one-way ANOVA (Kruskal-Wallis). A P value of < 0.05 was considered to be statistically significant (*P < 0.05, ** P < 0.01, and *** P < 0.001 vs control (Ctrl)). Scale bar: 15 μm