Fig. 3
Effect of increased intracellular Ca2+ concentration by CaEP on actin filaments in EA.hy926 and HMEC-1 cells. A Representative confocal images show changes in the F-actin cytoskeleton of both EA.hy926 and HMEC-1 cells at 1, 4 and 24 h after EP alone or after CaEP in the presence of 0.5, 2 or 3 mM Ca2+. B The plots represent quantification of changes in the average F-actin mean fluorescence intensity after CaEP at 1, 4 and 24 h time points. Data were normalized to the control and presented as AM ± SEM (n ≥ 3). Statistical significance was determined by a nonparametric one-way ANOVA (Kruskal-Wallis). A P value of < 0.05 was considered to be statistically significant (*P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs control (Ctrl)). Scale bar: 15 μm