Fig. 10

Comparison of the Ca²⁺ signaling-related transcriptomes of EA.hy926 and HMEC-1 cells. A Heatmap showing unsupervised hierarchical clustering of Ca²⁺ signaling-related genes across EA.hy926 and HMEC-1 samples. Normalized expression levels were scaled to Z-scores for each gene. B Volcano plot presenting DEGs related to Ca²⁺ signaling in EA.hy926 samples compared to HMEC-1 samples. Genes with adjusted P values of < 0.05 and |log₂FC| > 1 were considered to be statistically significant. A total of 72 genes were tested. NS - not significant, FC - fold change. C Bar plot showing the expression of genes related to channels involved in Ca²⁺ influx and efflux. A P value of < 0.05 and |log₂FC| > 0 were considered to be statistically significant (red – upregulated genes, blue – downregulated genes, ns – not significant). Dotted horizontal lines mark log₂FC > 1 and log₂FC < − 1. D-F Dot plots presenting the top 10 significantly enriched GO processes and four significantly enriched GO processes related to Ca²⁺ signaling based on hitsPerc (n (DEGs in category)/n (all genes in category) * 100). A P value < 0.05 was considered significant. BP - biological processes (D), CC - cellular components (E), MF - molecular functions (F). Five replicates per group in all analyses are presented