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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Characterization of two distinct immortalized endothelial cell lines, EA.hy926 and HMEC-1, for in vitro studies: exploring the impact of calcium electroporation, Ca2+ signaling and transcriptomic profiles

Fig. 1

Endothelial cells EA.hy926 and HMEC-1 after electroporation. A Untreated monolayers of EA.hy926 and HMEC-1 cells stained with Giemsa stain. Scale bar: 200 μm. B Permeabilization of ECs after exposure to electric pulses with increasing voltage. Fluorescence intensity of propidium iodine (PI) normalized to the control (0 V) 1 h after electroporation of EA.hy926 and HMEC-1 cells. The dotted vertical line marks the voltage of 645 V (1000 V/cm), which results in equivalent permeabilization of both cell lines. C Cell survival of ECs after exposure to electric pulses with increasing voltage. Normalized cell survival of EA.hy926 and HMEC-1 cells in suspension 72 h after exposure to electric pulses with increasing electric field. The values are presented as AM ± SE (n ≥ 40). Statistical significance was determined by a t-test. A P value of < 0.05 was considered to be statistically significant (*P < 0.05 vs control (Ctrl) (0 V), **P < 0.05 between EA.hy926 and HMEC-1 cells)

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Cell Communication and Signaling

ISSN: 1478-811X