Fig. 3

DNase-I alleviated the carcinogenesis of NETs in a CCA mouse model. A H&E and CK19 staining of AKT/YapS127 mouse for indicated group, Scale bar=50μm. B MPO-DNA levels analyzed by ELISA kit were much higher in WT mice compared with PAD4 KO mice. C Representative bioluminescence imaging of the transposon-based CCA model in WT mice and PAD4 KO mice. Photos were taken 21days after the hydrodynamic liver injection. D The weight of livers in the indicated group. E Representative gross images and HE staining of liver sections from WT mice and PAD4 KO mice. F and G Quantification of tumor burden in the indicated group, as determined by tumor number, tumor maximum size and Ki67 positive tumor cells. Data are means ± SD of three independent experiments. ***P<0.001