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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: The scaffold of neutrophil extracellular traps promotes CCA progression and modulates angiogenesis via ITGAV/NFκB

Fig. 2

NETs facilitated proliferation metastasis of CCA cell lines. A Proliferation rate was analyzed by a CCK-8 assay of indicated CCA cells treated with NETs or vehicle (5ng/μl). B Images of isolated cell cluster and growing organoids. Proliferation rate was analyzed by a CCK-8 assay of cholangiocarcinoma organoids cocultured with NET-DNA or vehicle (5ng/μl). C Transwell migration and invasion assays for indicated cell lines treated with NETs or vehicle (5ng/μl, 18h). D Optical tomography images of morphological changes in RBE cells induced by NET-DNA (5ng/μl, 24h). E qPCR analyzed the expression of E-cadherin, vimentin and Twist in RBE and KMBC cells treated with NET-DNA or vehicle (5ng/μl, 24h). Data are means ± SD of three independent experiments. *P<0.05, **P<0.01, ***P<0.001

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Cell Communication and Signaling

ISSN: 1478-811X