Fig. 5From: GOLGA7 is essential for NRAS trafficking from the Golgi to the plasma membrane but not for its palmitoylationGOLGA7 regulates anterograde trafficking of NRAS away from the Golgi (A) Anterograde trafficking of NRAS away from the Golgi was assessed via photoactivation of paGFP-NRAS on the Golgi (marked by mCherry-NRAS) and monitoring fluorescence loss over time; a representative cell is shown. Bars indicate 46.2 µm. B Curves trend the loss of initial fluorescence post-photoactivation in the region of the Golgi in cells ± sgGOLGA7 as mean ± SD for cells examined. Values are the means ± SD from n = 10 per group. C Retrograde trafficking of NRAS to the Golgi was examined using GFP-NRASG12D expressed in HeLa cells with FRAP kinetics determined in the region of the Golgi; a representative cell is shown. Bars indicate 46.2 µm. D Recovery curves trend the mean ± SD of the calculated fraction of initial fluorescence over time in the cells examined. Values are the means ± SD from n = 10 per groupBack to article page