Fig. 3

GOLGA7 knockout leads to elevated NRAS co-localization with the cis-Golgi (A, B) Immunofluorescence showing the colocalization of GFP-NRAS^G12D and the Golgi (A) or recycling endosomes (B) in HeLa cells ± sgGOLGA7. Scale bars, 42.6 µm. C Immunofluorescence showing the colocalization of GFP-NRAS^G12D and GIANTIN (cis and medial compartment of the Golgi, the upper panel) or TGN46 (trans compartment of the Golgi, the bottom panel) in HeLa cells ± sgGOLGA7. Scale bars, 42.6 µm (D) Statistical quantification of the colocalizations (Pearson’s coefficient) of GFP-NRAS.^G12D and GIANTIN or TGN46. Values are the means ± SD from n = 4 per group, compared using two- tailed Student’s t test. GIANTIN, ***p = 0.0001; TGN46, ns p = 0.0551