Fig. 2

GOLGA7 knockout does not disturb the plasma localization of HRAS, KRAS4A and KRAS4B (A-C) Immunofluorescence showing the colocalization of the plasma membrane and GFP-HRAS^G12D (A), GFP-KRAS4A^G12D (B) and GFP-KRAS4B^G12D (C) in HeLa cells ± sgGOLGA7. The intensity profiles of GFP-HRAS^G12D (A), GFP-KRAS4A^G12D (B) and GFP-KRAS4B^G12D (C) / the plasma membrane were plotted in the right panel. Scale bars, 42.6 µm. D-F Statistical quantification of the colocalizations (Pearson’s coefficient) of GFP-HRAS^G12D/GFP-KRAS4A^G12D/GFP-KRAS4B^G12D and the plasma membrane. Values are the means ± SD from n = 15 per group, compared using two- tailed Student’s t test. GFP-HRAS^G12D GOLGA7-sg1, ns p = 0.9519; GFP-HRAS^G12D GOLGA7-sg2, ns p = 0.3184; GFP-KRAS4A^G12D GOLGA7-sg1, ns p = 0.3668; GFP-KRAS4A^G12D GOLGA7-sg2, ns p = 0.1120; GFP—KRAS4B^G12D GOLGA7-sg1, ns p = 0.6088; GFP—KRAS4B^G12D GOLGA7-sg2, ns p = 0.4221