Fig. 4

MscL-G22S activation increases the glycolysis and OXPHOS in SCs. A Mitochondrial and glycolytic ATP production rates were measured using real-time ATP Rate Assay. B-E Extracellular and acidification rate (ECAR) and mitochondrial oxygen consumption ratio (OCR) were determined by Seahorse experiments (B, D). Quantitative analysis of glycolysis rate, glycolytic capacity, and glycolytic reserve in ECAR (C) and basal respiration, ATP production, maximal respiration, and spare respiratory capacity in OCR (E) demonstrated that the levels of energy metabolism were significantly upregulated in MscL-G22S-expressing SCs after mechanical stretching when compared to the controls. 2-DG, 2-deoxy-glucose; FCCP, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; R&A, rotenone/antimycin A; NC, negative control; MscL, MscL-G22S-expressing SCs; NC-stretch, negative control with mechanical stretching; MscL-stretch, MscL-G22S-expressing SCs with mechanical stretching. ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001