Fig. 2

Proteomic analysis of the effects of MscL-G22S activation on energy metabolism in SCs. Data of proteomic profiles analysis of SCs with different treatments were shown as heat maps, volcano plot, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. A The scaled expression value (row Z score) is shown as an orange-green color scheme where orange suggests for higher expression and green suggests for lower expression. B A series of differentially expressed proteins (DEPs) were identified between SCs with different treatment (fold change ≥1.2 or ≤ − 1.2; p < 0.05). In the plot, “down” means down-regulated DEPs, “up” means up-regulated DEPs and “unchange” means the difference was not statistically significant. The GO (C) and KEGG enrichment analysis (D) showed that the DEPs among SCs with different treatments were associated a variety of biological process, especially the biosynthesis and metabolism. NC, negative control; MscL, MscL-G22S-expressing SCs; NC-stretch, negative control with mechanical stretching; MscL-stretch, MscL-G22S-expressing SCs with mechanical stretching