Fig. 4

SHP1 knockdown dampens P5C effect on T cells. A After SHP1 knockdown, PKM2 and LDHB were captured from Jurkat cells treated with P5C by co-IP. Western bolt showing whether PKM2 and LDHB bind with each other in the absence of SHP1. B After SHP1 knockdown, western bolt showing the protein expression of PKM2 and p-PKM2 in Jurkat cells treated with P5C. An antibody to GAPDH was used as a loading control. C After SHP1 knockdown, Jurkat cells were treated with P5C for 24 h. Shown is the relative activity of PKM2. D After SHP1 knockdown, Jurkat cells were treated with P5C for 24 h. Shown is the relative intracellular lactate levels. E After SHP1 knockdown, Jurkat cells were treated with P5C for 24 h. Shown is the relative extracellular lactate levels. F After SHP1 knockdown, Jurkat cells were treated with P5C, and ECAR assays were performed using the Seahorse XF24 analyzer. G After SHP1 knockdown, IF showing the location of PKM in Jurkate cells. H Western blot showing the total, cytoplasmic and nuclear protein expression of PKM2 in Jurkat cells upon SHP1 knockdown. Error bars are SEM of biological replicates and ^***p < 0.01