Fig. 2

SHP1, PKM2 and LDHB bind with each other and P5C inhibits T cell glycolysis. A PKM2 was captured from Jurkat cells after treated with P5C, and all the proteins bound with PKM2 were checked by LC/MS-MS. All the proteins bound with PKM2 were summarized and shown in GO term and KEGG pathway. B SHP1, PKM2 and LDHB were pulled down by co-IP, and these three proteins bound with each other were shown by western bolt. C Western bolt showing the protein expression of PKM2 and p-PKM2 in Jurkat cells after treatment with PCM or P5C. An antibody to β-actin was used as a loading control. D Jurkat cells were treated with PCM or P5C for 24 h. Shown is the relative activity of PKM2. E Jurkat cells were treated with P5C, and ECAR assays were performed using the Seahorse XF24 analyzer. F Jurkat cells were treated with PCM or P5C for 24 h. Shown is the relative intracellular lactate levels. G Jurkat cells were treated with PCM or P5C for 24 h. Shown is the relative extracellular lactate levels. H Jurkat cells were treated with PCM or P5C for 24 h. Shown is the relative activity of LDH. Error bars are SEM of biological replicates and ^***, ^###p < 0.01