Fig. 1

MyD88 expression in myofibroblasts was associated with NAFLD-related liver cancer.  C57BL/6 mice were used for NAFLD model ( n  = 5 per group) and liver cancer model ( n  = 10 per group). A Schematic representation of the HFD induced NAFLD model. B Representative staining of H&E, Oil red O, and double staining of α-SMA and MyD88 in normal liver and NAFLD samples. Scale bar, 50 μm. C Schematic representation of the DEN/HFD induced liver cancer model. D Representative staining of H&E, Oil red O, and double staining of α-SMA and MyD88 in normal and HCC samples. Scale bar, 50 μm. E Statistical analysis (magnification, 200×). *** p  < 0.001. F The expression levels of MyD88 in liver tissues were determined by western blot. The densities of proteins were quantified using densitometry. Proteins were normalized to β-actin. *p<0.05, **p<0.01.  HFD: High fat diet; NAFLD: Non-alcoholic fatty liver disease; DEN: N-nitrosodiethylamine