Fig. 5
From: Egr-1 is a key regulator of the blood-brain barrier damage induced by meningitic Escherichia coli
Egr-1 is required for VEGFA, PDGFB, and ANGPTL4 induction. A ChIP-seq binding peaks on VEGFA, PDGFB, and ANGPTL4 gene promoter for Egr-1. B ChIP-PCR validation of the Egr-1 binding to the VEGFA, PDGFB, and ANGPTL4 promotor in hBMEC infected by meningitic E. coli. The nuclear extracts were immunoprecipitated with anti-Egr-1 antibody or normal IgG. Input refers to the same dose of nuclear extract administered prior to immunoprecipitation. C Real-time PCR analysis of VEGFA, PDGFB, and ANGPTL4 transcription in WT and Egr-1-knockout hBMEC upon infection. The transcription of β-actin was used as the internal reference. Data were presented as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001. D Western blot analysis of VEGFA, PDGFB, and ANGPTL4 expression in WT and Egr-1-knockout hBMEC upon infection. β-actin was used as the loading control, and densitometry was performed to analyze the differences