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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: Superbinder based phosphoproteomic landscape revealed PRKCD_pY313 mediates the activation of Src and p38 MAPK to promote TNBC progression

Fig. 8

DHE probe labeling and mito-tracker staining in TNBC cell lines with different PRKCD_pY313 level. (A) DHE probe labeling in stable transfected MDA-MB-231 cell lines with different levels of PRKCD_pY313 and quantification. Scale Bars = 100um. (B) DHE probe labeling in stable transfected BT549 cell lines with different levels of PRKCD_pY313 and quantification, Bar = 100um. (C) Mitochondrial membrane potential intensity in stable transfected MDA-MB-231 cells with different levels of PRKCD_pY313 without PMA and after treatment with 10 nM PMA for 20 min. Scale Bars = 10um. (D) Mitochondrial membrane potential intensity in stable transfected MDA-MB-231 cells with low PRKCD_pY313 level without PMA and after treatment with 10 nM PMA for 20 min. Scale Bars = 10um. (E) Mitochondrial membrane potential intensity in stable transfected BT549 cells with different levels of PRKCD_pY313 without PMA and after treatment with 10 nM PMA for 20 min. Scale Bars = 10um. PMA, Phorbol 12-myristate 13-acetate; DHE, Dihydroethidium; TNBC, triple negative breast cancer. Mean ± SD, n = 3 per group, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

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Cell Communication and Signaling

ISSN: 1478-811X