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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Superbinder based phosphoproteomic landscape revealed PRKCD_pY313 mediates the activation of Src and p38 MAPK to promote TNBC progression

Fig. 5

PRKCD_pY313 elevated the malignant biological behaviors of human TNBC cell lines. (A) The MTT assay in MDA-MB-231 and BT549 stable transfected TNBC cell lines with different PRKCD_pY313 level. (B) The colony formation rate of MDA-MB-231 and BT549 stable transfected cell lines with different PRKCD_pY313 level. (C) The apoptosis rate of MDA-MB-231 and BT549 stable transfected cell lines with different PRKCD_pY313 level, detected by flow cytometry. (D) The invasion assay of MDA-MB-231 and BT549 cell lines. (E) The quantification of wound healing assay of MDA-MB-231 and BT549 cell lines. (F) Western blot analysis of apoptotic related proteins (bcl-2, bax, cyto C), migration or invasion biomarkers (N-cadherin, Vimentin, β-catenin). All the results were presented from 3 independent experiments. TNBC, triple negative breast cancer; MTT, 3-(4,5)-dimethylthiahiazo (−z-y1)-3,5-di- phenytetrazoliumromide. Mean ± SD, n = 3 per group, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

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Cell Communication and Signaling

ISSN: 1478-811X